Cleavage of phosphorothioate-substituted DNA by restriction endonucleases.
نویسندگان
چکیده
منابع مشابه
Enzymatic Cleavage of Type II Restriction Endonucleases on the 2′-O-Methyl Nucleotide and Phosphorothioate Substituted DNA
The effects of nucleotide analogue substitution on the cleavage efficiencies of type II restriction endonucleases have been investigated. Six restriction endonucleases (EcoRV, SpeI, XbaI, XhoI, PstI and SphI) were investigated respectively regarding their cleavage when substrates were substituted by 2'-O-methyl nucleotide (2'-OMeN) and phosphorothioate (PS). Substitutions were made in the recog...
متن کاملStrand specific cleavage of phosphorothioate-containing DNA by reaction with restriction endonucleases in the presence of ethidium bromide.
A method for achieving strand specific nicking of DNA has been developed. Phosphorothioate groups were incorporated enzymatically into the (-)strand of M13 RF IV DNA. When such DNA is reacted with restriction endonucleases in the presence of ethidium bromide nicked DNA (RF II) is produced. All of the restriction enzymes tested linearised phosphorothioate-containing DNA in the absence of this dy...
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pBR322/Ultraviolet irradiation/Restriction endonuclease/Pyrimidine dimer Plasmid pBR322 DNA was irradiated with ultraviolet light at 254 nm and digested with various restric tion endonucleases. Analyses of the products suggested that the pyrimidine dimers which would have been formed just across the cutting site (Acc I), at the opposite position of the cutting site (Hind III, Eco RI, Bam HI), a...
متن کاملDNA polymorphism detectable by restriction endonucleases.
Data on DNA polymorphisms detected by restriction endonucleases are rapidly accumulating. With the aim of analyzing these data, several different measures of nucleon (DNA segment) diversity within and between populations are proposed, and statistical methods for estimating these quantities are developed. These statistical methods are applicable to both nuclear and nonnuclear DNAs. When evolutio...
متن کاملGeneration of DNA cleavage specificities of type II restriction endonucleases by reassortment of target recognition domains.
Type II restriction endonucleases (REases) cleave double-stranded DNA at specific sites within or close to their recognition sequences. Shortly after their discovery in 1970, REases have become one of the primary tools in molecular biology. However, the list of available specificities of type II REases is relatively short despite the extensive search for them in natural sources and multiple att...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1984
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)89884-2